Figure 3

Serosal cuticle is formed at complete germ band extension. Clarified eggs at different embryonic ages (item 6 of Methods): (A) 8-HAE embryo, showing the serosa anlage (arrowheads). (B, B') 9-HAE embryo at germ band extension, showing complete formation of serosa. (C) 13-HAE embryo at complete germ band extension. *: note posterior tip of germ band adjacent to the embryonic head. (D, D') 15-HAE embryo at the beginning of germ band retraction. In B' and D', colors aim to better visualize embryo surrounding layers. In D', the serosa is located bellow the serosal cuticle. Bar = 100 μm