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Figure 1 | BMC Developmental Biology

Figure 1

From: Embryonic desiccation resistance in Aedes aegypti: presumptive role of the chitinized Serosal Cuticle

Figure 1

Abrupt acquisition of desiccation resistance in Aedes aegypti embryogenesis is related to serosal cuticle formation. Pools of synchronized eggs at different embryonic ages were air-dried for 15 minutes (see items 2 and 3 of Methods). (A) The percentage of intact eggs was evaluated. Black arrow: end of embryogenesis. (B) 11-HAE and (C) 13-HAE eggs after 15 minutes air-exposure. The abrupt acquisition of impermeability between 11 and 13 hours of development is coincident with the appearance of the serosal cuticle, as determined by chlorine digestion (item 5 of Methods). (D) 11-HAE egg after 15 minutes exposure to chlorine. White dashed arrows: extrusion of cellular contents. (E) 13-HAE egg exposed to chlorine for 30 minutes. Note chorion almost complete disintegration. Black arrow: transparent serosal cuticle around the embryo. White arrows: reminiscent chorion.

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