Figure 8

Interaction of Tle2 with selected factors. Immunoprecipitations were performed by incubating 200 ug of Min6 cellular lysate with antibodies against Tle2 (+) or IgG (-). Lysate lanes containing whole cell extracts were included as a western blot control. Blots were probed with antibodies against endogenously expressed (A) Nkx2-2 or (B) Nkx6-1. (C) Immunoprecipitations using antibodies against Hes1 (+) or IgG (-) with subsequent blotting with antibodies against Tle2. This was done since the Hes1 band was obscured by the IgG heavy chain band in anti-Tle2 immunoprecpitations with subsequent anti-Hes1 blotting. In (D) an Arx cDNA containing a C-terminal 6xHis tag was transiently transfected into Min6 cells, due to the lack of endogenous Arx expression in these cells, 48 hrs prior to the IP and the resulting immunopreciptate probed with an anti-6xHis antibody.