Figure 4

Pax2 protein expression in the mouse forebrain at E14.5 and E16.5. A, a: Low and high power images of an E14.5 sagittal section showing strong Pax2 staining in the neuroepithelium of the septum (Se) adjacent to the foramen of Monro (FM) in an E14.5 sagittal section. This intense Pax2 staining is observed at the level where the lamina terminalis (LT) joins the septum. a shows a higher magnification of the boxed area in panel A. B, b: In more lateral sagittal sections Pax2 expression is found in a cell population (arrows) within the anterior hypothalamus (AH) reaching the medial horn (mh) of the lateral ventricle. b shows a higher magnification of the boxed area in panel B. C: E16.5 coronal section showing Pax2 expression around the optic chiasm (oc) region, in the optic stalk epithelium and in a few cells of the hypothalamic neuroepithelium next to the suprachiasmatic nucleus (SCH) (arrows). D: E16.5 coronal section showing Pax2 expression in the differentiating layer of the medial septum surrounded by the axonal bundles of the fornix (fx). Scale bars: A, B, 1000 μm; a, C, D, 200 μm; C-inset, 250 μm; b, 100 μm.