Figure 3

Pax2 is primarily expressed in neural progenitors in the septum and in early differentiated neurons in the hypothalamus. A: Double immunofluorescence with Pax2 (red) and β-tubulin III (Tuj1) (green) on a sagittal E12.5 telencephalic section shows that only a minority of Pax2-positive cells in the septum also express Tuj1, an early marker of differentiated neurons. This is further confirmed with double immunohistochemistry with Pax2 (black) and nestin (brown) (B), revealing that these Pax2-positive cells have nestin-positive filaments. Panels (A) and (B) show correspond to high power images taken within the region depicted in Fig. 2D, d. In the hypothalamus (C, D), most Pax2-positive cells express the early neural marker β-tubulin III (green), showing that they correspond to early generated neurons. Pax2-positive cells in (C) correspond to those shown in Fig. 2b', and those in (D) correspond to the cells indicated by arrowheads in Fig. 2b. The position of the ventricular zone (VZ) is indicated in sections A, C and D. Scale bars: A, 20 μm; B-D, 5 μm.