Figure 6

Depletion of XCIRP causes reduction of mRNA. (A) XCIRP is not necessary for convergent extension movements. Similar to dorsal animal zone explants of control embryos, more than 60 percent of the explants of CIRP-Mo injected embryos elongate. 0 and 1 indicate non-elongated explants, 2 indicate elongated and restricted explants and 2X elongated, but not constricted explants according to Schambony and Wedlich [27]. (B, C) Depletion of XCIRP results in a reduction of mRNA. Total RNA of stage 16 embryos was reverse transcribed either with oligo dT primer (mRNA) or random hexamer primer (total RNA) in the presence of 60 μCi P32 α-dCTP. (B) Shows an autoradiograph of a representative gel with a smear of labeled cDNA, the free radioactive nucleotides and the background. (C) The signals were quantified by setting the relation of mRNA/total RNA for the control as 1. Shown is the average and standard error for 3 independent experiments.