Figure 6From: Effects of NGF, NT-3 and GDNF family members on neurite outgrowth and migration from pelvic ganglia from embryonic and newborn miceConfocal images of transverse sections through the pelvic region at the level of the pelvic plexus primordium of E11.5 TH-GFP mice processed for Hu (blue), GFP (TH, green) and either GFRα1 (red) (A-D), GFRα2 (red) (E-H) or TrkC (red) (I-L) immunohistochemistry. Chains of Hu+ cells were present in the pelvic plexus primordium (A, E, I). A sub-population of the Hu+ cells was also GFP+ (TH+) (A-B, E-F, I-J). GFRα1 immunoreactivity was present on Hu+/TH-negative neurons, as well as some TH+/Hu+ neurons and some cells in the clusters that were TH-/Hu- (arrows) (A-D). Weak GFRα2 immunostaining was found on all Hu+ cells in the pelvic plexus primordium; however, strong GFRα2 immunoreactivity was found on many of the Hu+/TH+ cells (asterisks), whereas GFRα2 immunorectivity was weak on the Hu+/TH-negative sub-population (E-H). TrkC immunostaining was mainly observed on the Hu+/TH+ sub-population (I-L). Scale bars = 20 μm.Back to article page