Figure 1

Quantification of the effects of glial cell line-derived neurotrophic factor (GDNF), neurturin, artemin, NGF and NT3 on non-TH+ (area of Tuj-1 immunostaining minus area of TH immunostaining; A, C, E) and area of TH+ (B, D, F) fibre outgrowth from pelvic plexus explants taken at embryonic day (E) 11.5 (A-B), embryonic day (E) 14.5 (C-D) and postnatal day (P) 0 (E-F) and cultured for 4 days. The data (mean ± SEM) show the immunostained area as a percentage of mean immunostained area of control explants at each developmental stage. Significant responses are indicated with asterisks (one way ANOVA followed by Dunnett's post-hoc test; * – p < 0.05, ** – p < 0.01). "n"s refer to the number of explants analysed.