Table 1 The effect of BRL on osteo/adipogenic differentiation outcomes in RC single cell-derived colonies
| Â | Â | Colony Number | ||||
|---|---|---|---|---|---|---|
| Â | Â | BRL | Total | |||
| Â | Â | - | + | Â | ||
| Â | (Total) | 25 | % | 43 | % | 68 |
CFU | ALP+ | 25 | 100 | 31 | 72 | 56 |
| Â | Oil red O+ | 0 | 0 | 3 | 7 | 3 |
| Â | ALP+/Oil red O+ | 0 | 0 | 9** | 21 | 9 |
| Â | ALP-/Oil red O- | 0 | 0 | 0 | 0 | 0 |
| Â | OCN+ | 22 | 88 | 27 | 63 | 49 |
| Â | Adipsin+ | 0 | 0 | 3 | 7 | 0 |
| Â | OCN+/Adipsin+ | 0 | 0 | 9** | 21 | 9 |
| Â | OCN-/Adipsin- | 3 | 12 | 4 | 9 | 7 |
Gene expression | PPARγ+ | 4 | 16 | 3 | 7 | 7 |
|  | C/EBPα+ | 6 | 24 | 5 | 12 | 11 |
|  | PPAR γ+/C/EBPα+ | 7 | 28 | 31* | 72 | 35 |
|  | PPARγ-/C/EBPα- | 8 | 32 | 4 | 9 | 12 |
|  | OCN+/Adipsin+/PPARγ+/C/EBPα+ | 0 | 0 | 9** | 21 | 9 |