Figure 4

BRL increases adipocyte colonies without affecting osteoblastic colony formation. RC cells plated at 500 cells/100 mm dish were fixed at the days indicated and stained for adipogenesis (oil red O) and osteogenesis (ALP). A, Typical single cell colonies stained with ALP/von Kossa (day 27). B, Total number of CFU-F per 35-mm dish in the presence and absence of BRL at the concentrations indicated (day 27). C, The proportion of total colonies that are oil red O⁺ and/or ALP⁺ colonies in RC cells treated with and without BRL. Note that the proportion of the colonies showing a double (osteoblast plus adipocyte) phenotype increased with time. Data are mean ± SD of triplicate samples; results are representative of three independent experiments. *p< 0.05 vs. vehicle.