Figure 3
Expression profiling of PPARs and C/EBPs in the presence or absence of BRL during osteoblast development in RC cell cultures. Total RNA was collected as described in Fig. 2. Real-time RT-PCR was carried out by using specific primer sets for PPARα,γ, δ/β, C/EBPα, δ and L32. The ratio was calculated against the values of vehicle at day 5 that was set at 1.0. Data are mean ± SD of triplicate samples; results are representative of three independent experiments. *p< 0.05 vs. time-matched values of vehicle.