Figure 7

Dpy-30L2 knockout sperm in the wild type female reproductive system. Sperm of wild type males were marked by Mst77F or protamineB fused to eGFP to follow its fate in the female reproductive system. Wild type females were allowed to mate with wild type or mutant males, the females were then dissected and checked for a GFP signal in their reproductive system. 30 minutes after mating, 20% of wild type sperm had accumulated in female storage organs. However, mutant sperm remained in the uterus and failed to be transferred to seminal receptacle and spermathecae, the female sperm storage organs. Ov – ovaries; spt – spermatheca; src – seminal receptacle; ut – uterus; ovd – oviduct.