Figure 6
Transition of histones to protamines in dpy-30L2 knockout males. Degradation of histones was checked in dpy-30L2 knockout (dpy-30L26-1) males carrying a transgene with a fusion of GFP to the coding sequence of the histone H2A D variant (H2AvD). Arrow, H2AvD-GFP in degradation; arrowheads, H2AvD-GFP degraded. Incorporation of protamine B and Mst77F was analyzed in dpy-30L2 knockout males that carry either a transgene of protamine B fused to eGFP or of Mst77F fused of eGFP. During the "canoe" and "post-canoe" stages of spermatid development, ProtamineB-eGFP and Mst77F-eGFP incorporation in the spermatid nucleus appeared to be normal in dpy-30L2 knockout males. Any (diffuse) YFP signal from the dpy-30L2 promoter was not filtered out.