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Figure 2 | BMC Developmental Biology

Figure 2

From: GATA4/FOG2 transcriptional complex regulates Lhx9 gene expression in murine heart development

Figure 2

Quantitative analysis of Lhx9 gene expression in the control and Fog2 null hearts. A. Agarose gel electrophoresis showing RT-PCR products corresponding to the Lhx9α and Lhx9β isoforms of the Lhx9 gene expressed in the E11.5 tissues. The products corresponding to the cardiac tissue (lanes 1 and 5) were isolated and sequenced. Relative cardiac expression of the α(2.6) and β(1.0) Lhx9 isoforms in the wild-type sample was determined by the real time PCR. (B-C). Lhx9α/β is downregulated (~7-fold) while Fog2 is upregulated in the wild-type E9.5–E14.5 heart as demonstrated by the real-time PCR analysis of the pan-cardiac Lhx9α/β (B) and Fog2 (C) expression. Lhx9α/β is not expressed in the E9.5 embryonic heart. (D). A comparative analysis of Lhx9 gene expression in the control and Fog2 mutant E13.5 hearts using Lhx9-HD and Lhx9α/β isoform-specific primers. The Lhx9 expression for both isoforms is normalized to the Gapdh gene expression level; the fold changes (y axis) are shown; relative expression of Lhx9-HD is normalized to be 1.0. (E). A comparative analysis of the Lhx9-HD isoform expression in the E11.5 hearts and hind limbs. The Lhx9 expression is normalized to the Gapdh gene expression level; the fold changes (y axis) are shown; relative expression of Lhx9-HD in the heart is normalized to be 1.0. (F). A comparative analysis of Lhx9 gene expression in the E11.5 hind limb using Lhx9-HD and Lhx9α/β isoform-specific primers; relative expression of Lhx9-HD is normalized to be 1.0.

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