Identification of genes associated with regenerative success of Xenopus laevishindlimbs

Table 1 Effect of Noggin over-expression on limb regeneration following amputation at the future knee or ankle level.

Treatment	Type	Number of toes regenerated						N	Score/N	% regenerated	t	p
		0	1	2	3	4	5
Knee level amp.	WT	0	0	0	1	4	10	15	4.6	100
no heat shock	N1	0	0	2	2	2	10	16	4.3	100	1.08	0.293
Knee level amp.	WT	0	0	0	1	3	14	18	4.7	100
with heat shock	N1	16	5	1	2	1	0	25	0.7	36	15.20	<0.001
Ankle level amp.	WT	1	1	0	0	0	11	13	4.3	92
with heat shock	N1	10	1	0	0	1	0	12	0.4	16	6.71	<0.001

One limb bud was amputated (amp.) at the level of the future knee or ankle joint in anaesthetised tadpoles at NF stage 52. The contralateral limb was left intact. Where heat shock treatment is indicated, both WT and N1 tadpoles were heat shocked at -3 hrs, + 24 hrs and + 48 hrs relative to amputation time. The number of toes regenerated was recorded for each limb at stage 58. Two-sample t-tests were used to compare means, and significant difference was taken to be p > 0.05. There was no significant difference in regenerative ability between knee and ankle level amputations (t = 0.65, p = 0.524).

ISSN: 1471-213X