Age | Cre+/Srff/f | Cre+/Srff/+ | Cre-/Srff/f | Cre-/Srff/+ |
---|
E9.5 | 7 (0) | 5 | 10 | 16 |
E10.5 | 12 (0) | 14 (1) | 15 | 26 |
E11.5 | 19 (1) | 29 (2) | 7 | 17 |
E12.5 | 24 (11) | 21 | 17 | 7 |
E13.5 | 21 (19) | 15 | 14 (2) | 16 |
E14.5 | 10 (8) | 3 | 3 | 4 |
E18.5 | 2 (2) | 4 | 2 | 5 |
Neonate | 0 | 18 | 20 | 13 |
- Genotypic analysis of mouse embryos generated by crossing Tie2Cre+/-Srff/+ male mice with Srff/ffemale mice. Numbers represent total embryos of each genotype; numbers in parentheses indicate mutant phenotype and grossly necrotic embryos. The conditional loss of Srf gene function in endothelial cells resulted in embryonic death by E14.5; no animals with mutant genotype were detected during late gestational age or at birth.