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Figure 9 | BMC Developmental Biology

Figure 9

From: Endothelial-specific ablation of Serum Response Factor causes hemorrhaging, yolk sac vascular failure, and embryonic lethality

Figure 9

Tie2Cre+/-Srff/fyolk sac tissues show loss of blood vessel integrity and separation of dermal layers. Yolk sac tissues immunostained with VEC-specific marker PECAM-1 to label endothelial cells and vascular structures. Photographs of wild-type (A) and Tie2Cre+/-Srff/f(B) whole yolk sac at E13.5; arrowheads (B) mark apparent blood vessel remnants in mutant yolk sac tissue. Photomicrographs (200×) of cross-sections of wild-type (C) and Tie2Cre+/-Srff/f(D and E) yolk sac at E13.5 immunolabelled for PECAM-1 (brown-gray = PECAM-1, red = neutral red counterstain). Normal cellular layers of endoderm (Ed) and extraembryonic mesoderm (Md) are tightly associated in wild-type as compared to mutant yolk sac tissue. Asterisks mark areas of inappropriate tissue layer separation in Tie2Cre+/-Srff/fyolk sac (D). (E) Quantitation of laminar separation in cross-sections of yolk sac tissues as in C and D revealed increased delamination in mutant animals (16.3 ± 10.8% vs. 62.0 ± 10.4%, wild-type vs. mutant respectively; p = 0.0031). Scale bar: C, D = 50 μm.

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