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Figure 4 | BMC Developmental Biology

Figure 4

From: Yolk protein is expressed in the insect testis and interacts with sperm

Figure 4

YP2 protein contributes to yolk formation in the ovary of S. littoralis. (A) Western blot probed with YP2 antibody detected protein band at ~75 kDa in both seminal fluid (Lane 1) and ovarioles (Lane 2) of S. littoralis. Extracts of female fat body were immuno-negative (Lane 3). Equal amounts of total protein extracts (1 OD) were loaded in each lane. (B) Immunostaining of vitellogenic ovarioles of S. littoralis with YP2 antibody. Confocal microscopy detected staining in the oocyte cytoplasm (oc) and follicular cells (fc) that surround developing oocyte, but not in nurse cells (nc). (C) The YP2 signal was dramatically reduced when ovarian tissues were treated with antibody preabsorbed with fixed ovaries of P. interpunctella. Cell nuclei (blue) were stained with Hoechst 33258. Scale bar, 20 μm.

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