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Figure 2 | BMC Developmental Biology

Figure 2

From: Yolk protein is expressed in the insect testis and interacts with sperm

Figure 2

Yolk protein cross-reactive material is present in the male reproductive tract. Western blot with antiserum against YP2 from P. interpunctella detected a single protein band at ~75 kDa in seminal fluid of S. littoralis (Lane 1), and multiple bands at 75 – 80 kDa in extracts from P. interpunctella testis-UVD complexes (Lane 2) and ovarioles (Lane 3). The signal in S. littoralis seminal fluid was reduced when antibody was preabsorbed with P. interpunctella ovarian tissues prior to Western blotting (Lane 4). Equal amounts of total protein extracts (1 OD) were loaded in each lane.

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