Figure 7
Electroporation of pk1ΔPET/LIM affects the migration of primitive streak cells in vivo and in explants. (A) Quantitative representation of in vivo grafting experiments shown in Fig. 6. The normal behaviour of anterior and posterior explants, expressing GFP is represented as light grey shading. Dsh1-ΔPDZ/DEP disrupts normal behaviour anteriorly but not posteriorly. pk1-ΔPET/LIM and pk1 constructs disrupt normal behaviour posteriorly but not anteriorly. (B) Streak explants containing migratory cells are represented with black shading. Exposure of anterior explants to Wnt5a can induce migratory behaviour but this is abolished in explants expressing pk1ΔPET/LIM. The majority of posterior explants expressing pk1-ΔPET/LIM are no longer migratory and this cannot be rescued by exposure to Wnt-5a expressing cells. * asterisk indicates statistically significant results.