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Figure 6 | BMC Developmental Biology

Figure 6

From: Pax7 is requisite for maintenance of a subpopulation of superior collicular neurons and shows a diverging expression pattern to Pax3 during superior collicular development

Figure 6

Comparable astrocytic profile in the dorsal superior colliculus of wildtype and Pax7 mutant mice. Immunofluorescent detection of GFAP indicates a normal astrocytic profile at P5 (a-b) and P18.5 (c-d) for wildtype (a,c) and Pax7 mutant mice (b,d). (a') Positive control indicating GFAP expression in astrocytes of the myelencephalon. GFAP+ processes extend from cells located at the pial surface, however the dorsal half of the superior colliculus in Pax7-/- mice, like that of wildtype, is not populated by GFAP+ cells. Therefore, cell fate switching and/or transdifferentiation towards the astrocytic lineage does not account for the reduction in Pax7+ cells dorsally. Immunohistochemical detection of Pax6 (e, f, [inset from e]) and Engrailed (En-1) (g, h [inset from g]) was utilised to examine mesencephalic boundary formation, which appear morphologically unaffected in Pax7 mutant mice. Abbrev. Cc, cerebral cortex; Ipf, interpeduncular fossa;Mes, mesencephalon; Pt, pretectum; Ri, rhombencephalic isthmus. Scale bar: a-d,f,h 100 μm; e,g 500 μm.

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BMC Developmental Biology

ISSN: 1471-213X

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