Figure 5

Secretory activation does not occur in Src-/- mice following parturition. Histological sections were prepared from the mammary glands of Src+/+ (A-C), Src+/- (D-F), and Src-/- (G-I) mice at P18 (A, D, and G) and L2 (B, C, E, F, H and I). The sections shown in A, B, D, E, G and H were stained anti-ADRP antibody to outline the cytoplasmic lipid droplets (red), Oregon-Green 488-conjugated WPA to outline the surface of secretory alveoli (green), and DAPI to stain the nuclei of mammary epithelial cells (blue). Hematoxylin and eosin-stained sections of mammary glands at L2 are shown in C, F, and I. The bar in panel H represents 10 μm and the bars in panels C, F and I represent 100 μm. Luminal space is indicated by the letter "L", the white arrowheads indicate cytoplasmic lipid droplets, and the white arrows indicates regions where staining with WGA represents atypical patterns not observed in wildtype mice. The black arrows in panel I indicate large cytoplasmic lipid droplets not observed in wildtype mice.