Overexpression of Pax6 results in microphthalmia, retinal dysplasia and defective retinal ganglion cell axon guidance

Table 2

genotype	Retina area [μm² × 10⁶]	RGC layer depth [μm]	Density of Brn3a expressing cells in RGC layer [cells/μm³ × 10^-3]		Brn3a expressing cells/retina × 10³
			dn	vt
Pax6 ^Sey/+	1.74 ± 0.18	17.6 ± 1.4	7.9 ± 0.1	7.4 ± 0.6	241 ± 40
Pax6 ^+/+	2.90 ± 0.24	16.4 ± 1.6	8.9 ± 1.1	8.4 ± 0.3	405 ± 41
PAX77 ⁺	1.87 ± 0.24	18.9 ± 3.0	7.5 ± 0.2	7.6 ± 0.2	260 ± 15
PAX77 ^+/+	1.86 ± 0.20	17.9 ± 3.9	8.3 ± 0.3	8.5 ± 0.2	269 ± 28

Numbers and distributions of Brn3a expressing RGCs in Pax6^Sey/+, Pax6^+/+, PAX77⁺, and PAX77^+/+ embryos. Note that the reduced number of Brn3a-expressing cells in the Pax6^Sey/+, PAX77⁺, and PAX77^+/+ embryos compared to Pax6^+/+ embryos (see Fig 7 A) appears to correspond to a reduction in retinal area [that is having smaller eyes] as opposed to changes in the density or distribution of Brn3a-expressing cells in the retina. All values mean ± s.e.m. Sample sizes: Pax6^Sey/+n = 5; Pax6^+/+ n = 4; PAX77⁺ n = 3; PAX77^+/+ n = 3. Abbreviations: dn, dorsonasal quadrant; vt, ventrotemporal quadrant.

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