Figure 3

Pax6 o verexpression does not significantly affect retinal progenitor proliferation at E12.5 (A) Horizontal section of the eye of an E12.5 wild-type embryo labeled with anti-BrdU (red), anti-IdU (green) and counterstained with TOPRO3 (blue). Cell counts were made in 100 μm wide sampling boxes in the proximal (p) and distal (d) retina. (B) Estimated S-phase length (TS) and (C) estimated cell cycle length (TC) in the proximal and distal retina of wild-type and PAX77^+/+ E12.5 embryos. No significant differences were detected between the mutants and the wild-types (n = 3 of each; Student's t-test; p > 0.05). Scale bar: 100 μm.