Expression profiling in transgenic FVB/N embryonic stem cells overexpressing STAT3

Table 2 Upregulated genes

Probe set	Identifier	RefSeq	Chr	MM/PM fold change	MM/PM p-value (t-test)	PM fold change	PM p-value (t-test)	RTQ-PCR fold change
98524_f_at	X-linked myotubular myopathy gene 1 (Mtm1)	NM_019926	X	1.87	0.018906	1.91	0.017234	n.d.
94375_at	Hexokinase 2 (Hk2)	NM_013820	6	1.6	0.018958	1.61	0.015899	1.79 ± 0.11
95545_at	Insulin-like growth factor 1A (Igf1)	NM_010512	10	2.34	0.003079	1.6	0.007358	n.d.
98059_s_at	lamin A (Lmna)	NM_001002011	3	1.58	0.070634	1.47	0.035881	n.d.
95562_at	Nuclear domain 10 protein 52 (Ndp52)	NM_029755	11	2.66	0.065377	2.08	0.057843	6.72 ± 0.67
101368_at	Reproductive homeobox 5 (Rhox5/Pem)	NM_008818	X	1.68	0.029649	1.82	0.013529	3.64 ± 0.3
103470_at	Pramel6	NM_178249	2	6.18	0.02356	4.14	0.027034	10.0 ± 0.37
93245_at	Pramel7	NM_178250	2	3.51	0.051948	2.77	0.052927	8.51 ± 0.92
95609_at	Protein phosphatase 1, regulatory (inhibitor) subunit 15b (Ppp1r15b)	NM_133819	1	1.6	0.024109	1.5	0.022545	1.56 ± 0.12
92232_at	suppressor of cytokine signaling 3 (Socs3)	NM_007707	11	1.75	0.053978	1.56	0.015837	2.34 ± 0.35
101449_at	tripartite motif-containing 41 (Trim41)	NM_145377	11	-	-	4.11	0.000819	1.07 ± 0.17
160365_at	Eukaryotic translation initiation factor 2, subunit 2 beta (Eif2s2)	NM_026030	2	1.98	0.040383	1.96	0.042002	1.80 ± 0.09
160420_r_at	tubulin, alpha 3A (Tuba3a)	NM_009446	6	2.07	0.080906	1.93	0.078476	n.d.

Genes upregulated after STAT3-MER activation through OHT. Microarray values are given as fold changes ratios relative to normalization derived from dCHIP. 13 genes were found to be significantly upregulated when compared with WT FVB/N cells cultivated in presence of LIF. Only genes with a 1.5× fold increase were considered as significant. The mRNA expression levels (mean ± standard deviation, n = 3) of relative to FVB WT control cells were validated by real time PCR analysis, RTQ-PCR values are ratios relative to β-actin (housekeeper gene) expression. For all selected genes measured, RT-PCR corroborated the rank order of magnitude of expression measured on the microarrays. Calculations were performed with both the PM/MM (perfect match/mismatch) difference model and the PM (perfect match) only model in order to compare the results.

ISSN: 1471-213X