Figure 7
From: Divergent roles for Eph and Ephrin in Avian Cranial Neural Crest
Distribution of EphB2 mRNA and HNK-1 protein in the embryonic avian hindbrain. Wholemount (a-c) and cross-section (d-f) images of a stage 14 chicken embryo double labeled for EphB2 and HNK-1. (a-c) EphB2 probe staining is in patches (arrows) bordering cells labeled by HNK-1. Inset shows embryo at lower magnification. The margins of the regions expressing EphB2 have been traced with a thin white line in panel a. The tracing from panel a has been superimposed on the image in panel b to show the margins relative to the HNK-1 expressing cells. (d-fi) In cross-section (taken at black line in c), probe (arrow) and HNK-1 stained cells have a non-overlapping distribution. Inset shows section at lower magnification. Ec, ectoderm; My, mesenchyme; s, somite.