Figure 6

Distribution of ephrin-B2 mRNA and HNK-1 protein in the hindbrain region of the stage 12–14 embryos. At stage 14, ephrin-B2 probe labels streams of cells rostral and caudal to the otic vesicle (arrows), as does HNK-1 (a, b). The probe has a similar distribution in stage 13 (c) and stage 12 (d) embryos. Insets show the same embryos at lower magnification. In cross section, ephrin-B2 probe binding cells again co-localize with cells stained by HNK-1 (arrowheads) (e, f). Inset shows the same section at lower magnification. BA, branchial arch; Ec, ectoderm; My, mesenchyme; NT, neural tube; OV, otic vesicle. Distribution of ephrin-B2 mRNA and HNK-1 protein in the embryonic avian hindbrain. Wholemount (a, b) and cross-section (c, d) images of a stage 14 chicken embryo double labeled for ephrin-B2 and HNK-1. (a, b) Ephrin-B2 probe labels cells rostral and caudal to the OV (arrows), as does HNK-1. Inset shows embryo at lower magnification. (c, d) In cross-section, ephrin-B2 probe binding cells again colocalize with cells stained by HNK-1 (arrowheads). Inset shows section at lower magnification. Ec, ectoderm; My, mesenchyme; s, somite.