Skip to main content
Figure 5 | BMC Developmental Biology

Figure 5

From: Divergent roles for Eph and Ephrin in Avian Cranial Neural Crest

Figure 5

The distribution of ephrin-B1 mRNA and HNK-1 protein in the embryonic avian hindbrain. Wholemount (a, c, e) and cross-section (b, d, f) images of a stage 14 chicken embryo double labeled for ephrin-B1 and HNK-1. Inset shows low magnification image of staining in somites of this embryo. (a-c) The stream of CNC cells rostral to the OV comes to an end at the point where strong ephrin-B1 probe staining appears in BAII (arrow 1). The margins of the regions expressing ephrin-B1 have been traced with a thin white line in panel a. The tracing from panel a has been superimposed on the image in panel c to show the margins relative to the HNK-1 expressing cells. (c – f) The CNC stream caudal to the OV is bordered by groups of cells (arrows 2–4) labeled by the ephrin-B1 probe. In cross-section (taken at fine black line in e), probe (arrow) and HNK-1 stained cells have a non-overlapping distribution. BA, branchial arch; Ec, ectoderm; My, mesenchyme; NT, neural tube; s, somite; OV, otic vesicle.

Back to article page