Figure 10

FLI-1::GFP accumulates at germ line nuclei. Confocal images of gonads from animals stained with anti-GFP antibody (green) and DAPI to label nuclei (blue). (A-F) are images of gonads from wild-type animals harboring the full-length fli-1::gfp transgene that rescues the fli-1 Glm phenotype and that is predicted to encode a full-length FLI-1 protein tagged with GFP at the C-terminus. (G-I) are images of a wild-type animal without the fli-1::gfp transgene. (A-C) are images of the transition zone (upper right) to pachytene zone (lower left); (D-F) are images of the pachytene zone. FLI-1::GFP reactivity was found associated with germ line nuclei. The scale bars in A and G represent 5 μm for A-C and G-I. The scale bar in D represents 2 μm for D-F.