Figure 5

C. elegans dss-1 can partially rescue yeast sem1 mutation. A. S. cerevisiae sem1 mutants have a growth defect at restrictive temperature (vector). Expression of either C. elegans dss-1 or the yeast SEM1 in sem1 mutants rescues the growth defect. B. Accumulation of polyubiquitinated proteins is seen in sem1 mutants transformed with an empty vector both at permissive and restrictive temperature at two different time points. Expression of yeast SEM1 in sem1 mutant cells reduces efficiently the accumulation of polyubiquitinated proteins, whereas expression of C. elegans dss-1 has only a modest effect. Lower panels have been blotted with anti-Sso1/2 antibody as a control for equal loading.