Figure 4

dss-1 phenotype resembles both brc-2 and kgb-1 mutants. A. brc-2(tm1086) mutants are sterile with a uterus filled with abnormal embryos. B. Abnormal diakinesis occurs in brc-2 oocytes as shown by DAPI staining. C. dss-1 oocytes appear to have the expected number of six condensed chromosomes, but they occasionally show defects in diakinesis (D). In B-D only one focal plane is shown. E-M. Nomarski optics; E'-M' DAPI staining. E. kgb-1 mutants grown at 26°C lack embryos in the uterus (u). Their gonads are often disorganised (F, F', arrows indicate oocyte chromosomes) and similar disorganisation is sometimes seen in dss-1 homozygotes (G, G', arrow indicates an oocyte). DAPI-positive emo oocytes are often present in kgb-1 (H), and occasionally in dss-1 gonads (I). J. emo-like structures are also seen in dss-1 uteri. K-M. rpn-10 mutants grown at the restrictive temperature (25°C) have oocytes (K) but lack sperm (L, M). 15°C, permissive temperature. Asterisk, spermatheca; em, emo cell; g, germ cells; u, uterus; v, vulva.