Figure 7

dAux mutation disrupts clathrin distribution. Confocal images of (A-F) ey>FLP/UAS-EGFP-Clc; FRT^5-5Z3515, dAux^L78H/FRT^5-5Z3515, GMR-myr-mRFP and (G-N) ey>FLP/UAS-EGFP-Clc; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, GMR-myr-mRFP eye discs at two different optical planes (apical: A-C and G-J, and basal: D-F and K-N). For panels A-F, EGFP-tagged Clc, expressed in all cells, is shown in green, and dAux^L78Hmutant cells are indicated by the absence of a membrane-associated mRFP (myr-mRFP, shown in red). For panels G-N, EGFP-Clc is shown in green, Dl staining is shown in red, and dAux^F956* mutant cells are indicated by the absence of a membrane-associated myr-mRFP (blue). Arrows indicate the intense Clc-EGFP-positive structures around the cell periphery. The dAux^L78Hclone (A-F) is located in the posterior part of the eye disc, whereas this dAux^F956* clone (G-N) is located near the furrow. As a result, the anterior boundary of the dAux^F956* clone is not marked because GMR-myr-mRFP is only active in cells posterior to the furrow [85]. In the basal section of the dAux^F956* clone, less vesicular Dl staining was seen, compared to the nearby wild-type cells. Scale bar, 10 μm.