Figure 5

dAux mutant cells show an accumulation of Dl at the surface. Confocal images of (A-C and G-I) Act5C>FLP/+; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nlsand (D-F) Act5C>FLP/+; FRT^5-5Z3515, dAux^L78H/FRT^5-5Z3515, ubi-GFP^nlseye discs stained for Dl (red). In panels A through F, the Dl staining was performed in the presence of detergent. In contrast, the Dl staining in G- I was performed under non-permeabilized conditions, thereby detecting only Dl proteins at the cell surface. dAux mutant cells are indicated by the absence of nuclear GFP. Scale bar, 10 μm.