Figure 4

dAux functions in the signal-sending cells during Notch signaling. Projected spinning disk confocal images of (A-C) N^264-39, FRT^9-2/ubi-GFP^nls, FRT^9-2; Act5C>FLP/+, (D-F) ey-FLP/+; FRT82B, Dl^RevF10/FRT82B, ubi-GFP^nlsand (G-I) Act5C>FLP/+; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nlslarval eye discs stained with mAB323 anti-E(spl) antibody (red). N^264-39, Dl^RevF10 and dAux^F956* mutant cells are indicated by the absence of nuclear GFP, and the boundaries of mutant clones are outlined with dotted lines. Please note that all N^264-39 cells are negative for E(spl) staining, whereas some Dl^RevF10 and dAux^F956* cells at the clone boundaries are E(spl)-positive (indicated by arrows). Scale bar, 10 μm.