Figure 3

dAux mutations disrupt Cut expression at the DV boundary in developing wing discs. Fluorescent micrographs of (A) wild-type and (B-D) Act5C>FLP/+; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nlslarval wing discs stained with 2B10 anti-Cut antibody (red). A mutant clone at the DV boundary (indicated by the white box) is shown at higher magnification in the inset in (D). dAux^F956* mutant clones are indicated by the absence of nuclear GFP (C). Some dAux^F956* cells (indicated by arrows) at the clone boundary are Cut-positive (see text for explanation). Scale bar, 50 μm.