Figure 2

dAux mutations disrupt the proneural enhancement and the lateral inhibition during R8 specification. Projected spinning disk confocal micrographs of Act5C>FLP/+; FRT^5-5Z3515, dAux^F956*/FRT^5-5Z3515, ubi-GFP^nlslarval eye stained for Atonal (A-D, red) and Senseless (E-H, red). Regions marked with boxes in (C) and (G) are shown at a higher magnification in (D) and (H), respectively. dAux^F956* mutant clones are indicated by the absence of nuclear GFP and outlined with dashed lines in (D) and (H). dAux^F956* mutant cells with an elevated level of Atonal expression at the clone border are indicated by solid arrows (C and D). Furthermore, groups of Atonal-positive cells that fail to resolve into single Atonal-expressing cells are indicated by open arrows (A and D). Scale bar, 50 μm.