Age- and calorie-independent life span extension from dietary restriction by bacterial deprivation in Caenorhabditis elegans

Table 2 Transient BD has long-term survival benefits.

Cohort	Number of animals	Mean Survival After Transfer	Mean Control Fed Survival After Transfer	p-value
CF	57	-	20.5 ± 0.6	-
BD	97	34.8 ± 0.7	20.5 ± 0.6	1.7 × 10^-19
Day 8 BD to CF	50	24.4 ± 0.9	20.5 ± 0.6	3.0 × 10^-4
Day 14 BD to CF	36	28.3 ± 1.0	22.0 ± 0.6	2.0 × 10^-6
Day 28 BD to CF	117	35.7 ± 0.4	31.4 ± 0.4	0.03

'Mean survival after transfer' is the average life span of animals in each group that were alive at the time the cohort was switched from BD back to a control fed (CF) diet. 'Mean control fed survival after transfer' is the average life span of control fed animals that were alive at the time the corresponding cohort was switched to BD. Survival data are shown as mean ± standard error. P-values were calculated by the Wilcoxon Rank-Sum test using the MATLAB 'ranksum' function. Bacterial deprivation (BD) was initiated at the 2^nd day of adulthood.