Figure 1From: Age- and calorie-independent life span extension from dietary restriction by bacterial deprivation in Caenorhabditis elegansDietary restriction by bacterial food deprivation (BD) extends life span independent of age or duration. (A) BD increases survival when initiated at 4 (red line, square symbols), 8 (blue line, circles), 14 (green line, triangles), 20 (gray line, plus symbols), or 24 (purple line, astericks) days of adulthood, relative to control fed (CF) animals. Mean life spans ± standard error, statistical significance, and number of animals examined provided in Table 1. (B) Animals subjected to transient BD, from the day 2 of adulthood to day 8 (blue line, square symbols), 14 (purple line, triangles), or 28 (green line, circles) have increased survival relative to animals maintained on CF until death (black line, diamonds). Mean life spans ± standard error, statistical significance, and number of animals examined provided in Table 2. (C) Mean survival of animals in (B) alive when BD-treated animals were transferred back to control fed conditions shows a significant increase in survival of animals exposed to transient BD relative to animals maintained on a control fed diet for life.Back to article page