Figure 4

PKD3 expression at embryonic stage E12.5. Immunohistochemistry was performed on sagittal sections (6 μm) of E12.5 mouse embryos. (A) – (B) Control sections incubated without primary antibody. (C) – (L) Sections incubated with anti-PKD3 antibody (1:2000). bc: atrio-ventricular bulbar cushion tissue, cp: origin of choroid plexus, cv: cartilage primordium of vertebra, da: descending (thoracic) aorta, dt: dorsum of tongue, fv: fourth ventricle, ll: left lung, ls: lumen of stomach, lv: lateral ventricle, ml: marginal layer of spinal cord, mm: mucous membrane, nc: nasal cavity, not: notochord, np: nasopharynx, oe: olfactory epithelium, op: oesophagus, or: optic recess of diencephalon, pc: pericardial cavity, pl: pleural cavity, rg: root ganglia, sb: segmental bronchus, sc: spinalcord, tb: cartilage primordium of temporal bone, ul: upper lip, us: urogenital sinus, vo: vomeronasal organ. Scale bars: 100 μm.