PKD3 expression at embryonic stage E11.5. Immunohistochemistry was performed on sagittal sections (6 μm) of E11.5 mouse embryos. (A) – (B) Control sections incubated without primary antibody. (C) – (J) Sections incubated with anti-PKD3 antibody (1:2000). ac: atrial chamber, bs: cartilage primordium of base of the skull, C1: condensation of sclerotomic material forming centrum of atlas, cm: cephalic mesenchyme, da: dorsal aorta, ec: endocardial cushion tissue lining the atrio-ventricular canal, lb: lung bud, mb: main bronchus, nc: notochord, np: nasal process, op: olfactory pit, rh: roof of hindbrain, vh: ventricular chamber of heart. Scale bars: 100 μm.