Specificity of the PKD3 antibody. A: Sequence alignment of mouse PKD3 with human PKD3, mouse PKD1 and PKD2. The sequence of PKD3 used for peptide synthesis and immunisation of mice is marked in red. B: HEK293T cells were transfected with the indicated plasmids, whole cell lysates were prepared and Western blot analysis was performed with the indicated antibodies. C: Immunohistochemistry on sagittal sections (6 μm) of E14.5 mouse embryos. Sections incubated with mouse Flag- and rabbit GFP-specific antibodies (both 0.5 μg/ml), respectively or without primary antibody (-) were negative and used as control. Section incubated with rabbit anti-PKD3 antibody (1:2000) displayed specific staining.