Figure 4

Horizontal cell subtypes express different sets of markers. (A-O): The horizontal cell layer of flat-mounted P0 retinas labelled with Lim1 (A-E, green), Isl1 (F-J, green) and Ap2α (K-O, green), combined with Prox1 (A, F, K, red), Pax6 (B, G, L, red), GABA (C, H, M, red), calretinin (D, I, N, red) and TrkA (E, J, O, red). Double positive cells appear yellow. (P-S): Calculated fractions (expressed as % ± s.d., n animals = 3) of HCs co-expressing various markers with respect to Prox1 (P), Isl1 (Q), GABA (R) and calretinin (S). Asterisks in P denote that these fractions were calculated using known fractions of other markers. (T-U): Distribution of HC markers at P0 and P7. Scale bar is 20 μm (valid for A-O).