Figure 3From: Axon-bearing and axon-less horizontal cell subtypes are generated consecutively during chick retinal development from progenitors that are sensitive to follistatinConfocal analysis of horizontal cell morphology and changes in GABA expression levels after hatch. (A): Axon-bearing, brush-shaped HCs visualised by calretinin. (B): Flat-mounted P7 retina. GABA antibodies (red) label a fraction of Isl1+ HCs intensely (green, arrow, co-expression is yellow), and label Lim1+ HCs to a lesser extent (arrowhead). (C): Axon-less stellate HCs visualized by intense GABA labelling. (D): Axon-less candelabrum-shaped HCs visualised by TrkA. (E-G): Morphology of chick HCs as they appear in Gallego, 1986 [6] with names and molecular attributes denoted below. (H-K): P0 (H-I) and P7 (J-K) HCs labelled for GABA (H-K, red) and Lim1 (H and J, green). Uniform GABA levels are found in both Lim1+ and Lim1-HCs at P0 (compare h with h') whereas Lim1+ HCs express low levels of GABA at P7 (compare j with j'). Scale bars are 5 μm (valid for A-D and H-K).Back to article page