Figure 1From: Proliferating mesodermal cells in murine embryos exhibiting macrophage and lymphendothelial characteristicsJugular lymph sacs of murine embryos. Lyve-1 staining of paraffin sections (A, B) and double staining of cryo-sections (C-F) of ED 13.5 (A-C, E, F) and 12.5 (D) mice. A, B) The jugular lymph sacs (jls) are Lyve-1+ and located dorso-laterally of the cardinal vein (v). Many scattered Lyve-1+ cells are located superficially in the developing dermis (arrows). nt, neural tube. B) Higher magnification of A showing JLS in close proximity to the cardinal vein. C) Lymph sacs express Prox1 (red) and Lyve-1 (green). D) Prox1 (red) and LA102 (green). E) Lyve-1 (red) and LA102 (green). Note double positive LECs, although staining of lymph sac LECs with LA102 is heterogeneous. F) Lymphatic vessel (arrow) in the dermis is double positive for Lyve-1 and LA102 (yellow).Back to article page