Figure 2

U0126 produces a specific and consistent phenotype affecting pigmentation and notochord development. Phenotype of wild type and U0126-treated embryos at 24 hpf. (A-F) and at 48 hpf (G-L). There is a total lack of pigmentation including in the RPE in the eye (compare panels I and L), twists and undulations in the NC are already evident at 24 hpf. (D, E) and the trunk and tail of treated embryos is shortened at 48 hpf (compare panels G and J). The large yolk sac in 48 hpf embryos indicates that circulation is not established properly to carry away its content (see also later in Fig. 5) Panels A-C, G-I: wild type embryos. Panels D-F, J-L: U0126-treated embryos.