Figure 7

CNP-induced bone growth requires PI3K activity. (A) Tibiae isolated from E15.5 mice were cultured for 6 days in the presence of CNP, control (0.1% HCl-BSA in PBS), LY294002 or CNP + LY294002. Bone growth measurements showed increased bone growth in the CNP treatment, and no significant difference between the LY294002 and CNP+LY294002. (B) E 15.5 tibiae treated for 6 days with the treatments mentioned above, were fixed, embedded, sectioned and stained with H&E. The length of hypertrophic zone was increased in the CNP treatment compared to control, decreased in the LY294002 treatment and similar between LY294002 and CNP+LY294002