PI3K inhibition affects chondrocyte morphology. (A) E15.5 tibiae, cultured for 6 days in the presence of LY294002 or DMSO were stained with Safranin O for proteoglycan content. The chondrocyte morphology was analyzed by comparing the chondrocyte size in the three zones of the growth plate. We noticed a reduction of hypertrophic cell size in the case of hypertrophic chondrocytes. (B) E18.5 tibiae treated with LY294002 for 6 days showed the same reduction in hypertrophic zone length and cell size as the E15.5 treated tibiae.