Figure 9
From: Competition between Delta and the Abruptex domain of Notch
pH does not affect binding to the spl mutant. A. Dl:Fc bound to adsorbed N11-14:V5:His protein at pH7.4, B. Dl:Fc bound similarly to adsorbed N11-14spl:V5:His protein at pH7.4, C. Dl:Fc binding to adsorbed N11-14:V5:His protein was enhanced at pH5.4, D. Dl:Fc binding to adsorbed N11-14spl:V5:His protein was similarly enhanced at pH5.4, E. N21-30:Fc bound to adsorbed N11-14:V5:His protein at pH7.4, F. N21-30:Fc bound similarly to adsorbed N11-14spl:V5:His protein at pH7.4, G. N21-30:Fc binding to adsorbed N11-14:V5:His protein was not enhanced at pH5.4, H. N21-30:Fc bound similarly to adsorbed N11-14spl:V5:His protein at pH5.4,