Figure 5

Competition between Dl and the Abruptex region. A. Binding of 10 ng (2.74 nM) N21-30:Fc to N11-20 is competed by increasing amounts of N21-30:V5:His. B. Binding of 10 ng (2.74 nM) N21-30Fc to N11-20 is competed by increasing amounts of Dl:V5:His. As Dl does not bind to N21-30 (see Figure 2D), Dl:V5:His binding to N11-20 is the likely mechanism. C. Binding of 100 ng (23.2 nM) Dl:Fc to N11-20 is competed by increasing amounts of Dl:V5:His. D. Binding of 100 ng (23.2 nM) Dl:Fc to N11-20 is competed by increasing amounts of N21-30:V5:His. As Dl does not bind to N21-30 (see Figure 2D), Dl:V5:His binding to N11-20 is the likely mechanism.