Figure 6

Chemical enhancer of reg6 mutation. (A) reg6 is a temperature-sensitive mutation and regenerates almost normally at 20°C. Shown is the regenerating vessels 6 days post amputation at 20°C. Note that they regenerated much more slowly at the low temperature. (B) When treated with 300 μM of the histidine decarboxylase inhibitor, urocanic acid, reg6 siblings developed a significant number of swollen vessels (yellow arrows). Red arrows, amputation plane. (C) The enhancement by urocanic acid of reg6 vessel defects was evaluated at two regenerative stages, during anastomosis (3 dpa at 20°C, n = 10) and plexus formation (6 dpa at 20°C, n = 10) that are both affected by the reg6 mutation [16]. U300, 300 μM of urocanic acid.